Physicochemical properties and biological activity of novel cerium oxide nanoparticles modified with pyrroloquinoline quinone
https://doi.org/10.17586/2220-8054-2024-15-5-683-692
Abstract
Nanoscale cerium oxide (CeO2) is a bioavailable inorganic nanozyme exhibiting pronounced redox activity and capable of acting as a delivery system for bioactive compounds. We have synthesized and characterized novel CeO2 nanoparticles modified with pyrroloquinoline quinone (CeO2@PQQ). TEM analysis revealed the diameter of the CeO2@PQQ NPs to be approximately 4 nm, with a hydrodynamic diameter of 62 nm (DLS). Furthermore, the zeta potential was found to be −38 mV (ELS), and FTIR analysis confirmed the adsorption of PQQ on the surface of CeO2 NPs. The results demonstrated that CeO2@PQQ NPs exhibited no cytotoxic effects on L929 cells within the concentration range of 0.1 – 10 µM and did not adversely affect the mitochondrial function of the cells. It was demonstrated that CeO2@PQQ NPs exhibited protective effects against L929 cells when induced with oxidative stress (200 µM H2O2), leading to preservation of cell mitochondrial potential levels up to 76 % of control and cell viability up to 78 % before and after incubation with CeO2@PQQ NPs. The results indicate that CeO2@PQQ NPs can be regarded as a novel hybrid nanosystem that exhibits mitochondrial-directed control of oxidative stress.
About the Authors
Elizaveta A. Zamyatina
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
Russian Federation
Russian Federation
Elizaveta A. Zamyatina
Institutskaya str., 3, Pushchino, 142290
Olga A. Goryacheva
Saratov State University named after N. G. Chernyshevsky, Chemistry Institute
Russian Federation
Russian Federation
Olga A. Goryacheva
Astrakhanskaya 83, Saratov, 410012
Nelli R. Popova
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
Russian Federation
Russian Federation
Nelli R. Popova
Institutskaya str., 3, Pushchino, 142290
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Review
For citations:
Zamyatina E.A., Goryacheva O.A., Popova N.R. Physicochemical properties and biological activity of novel cerium oxide nanoparticles modified with pyrroloquinoline quinone. Nanosystems: Physics, Chemistry, Mathematics. 2024;15(5):683-692. https://doi.org/10.17586/2220-8054-2024-15-5-683-692
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