Физико-химические характеристики и биологическая активность новых наночастиц оксида церия, модифицированных пирролохинолинхиноном

Наноразмерный оксид церия (CeO₂) является биодоступным неорганическим нанозимом, проявляющим выраженную окислительно-восстановительную активность и способным выступать в роли системы доставки биоактивных соединений. Нами были синтезированы и охарактеризованы новые наночастицы (NPs) CeO₂, модифицированные пирролохинолинхиноном (PQQ). Показано, что CeO₂@PQQ NPs имеют размер до 4 нм (ТЭМ), гидродинамический диаметр составил 62 нм (DLS), дзета-потенциал -38 мВ, PQQ адсорбируется на поверхности CeO₂ NPs (FTIR). Установлено, что в диапазоне концентраций 0,1-10 мкМ CeO2@PQQ NPs не оказывают цитотоксического эффекта на культуру клеток фибробластов мыши (L929), а также не оказывают негативного влияния на митохондриальную функцию L929. Было выявлено, что при индукции окислительного стресса (200 мкМ H₂O₂) CeO₂@PQQ NPs проявляют защитное действие в отношении L929 в концентрациях 0,1-10 мкМ, приводя к сохранению уровня митохондриального потенциала клеток до 76% от контроля и жизнеспособности клеток до 78% до и после добавления наночастиц. Результаты работы позволяют рассматривать CeO₂@PQQ NPs в качестве новой гибридной наносистемы для митохондриально-направленной борьбы с окислительным стрессом.

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