Synthesis and biocompatibility study of ceria-mildronate nanocomposite in vitro
https://doi.org/10.17586/2220-8054-2022-13-1-96-103
Abstract
Nanoscale cerium dioxide (CeO2, nanoceria) possesses notable redox activity, which is actively used in advanced biomedical applications. The low toxicity, high biocompatibility and antioxidant activity of nanoceria make it a new generation nanozyme with a unique activity. Combination of nanoceria with various biologically active substances results in organic-inorganic nanocomposites possessing enhanced activity. Here, we synthesized a novel organic-inorganic hybrid material (Mil-CeO2) based on 2-(2-carboxylatoethyl)-1,1,1-trimethylhydrazinium and nanoceria, which has an ultra-small particle size, high antioxidant activity and pronounced biological activity. The analysis of cytotoxicity of the composite did not reveal any negative effects on the NCTC L929 mouse fibroblasts at concentrations below 10 mM. It was shown that the nanocomposite did not cause morphological changes in cells, or lead to cell death and mitochondrial membrane potential disruption, while maintaining viability in mouse fibroblasts in vitro. Additionally, we showed that Mil-CeO2 is capable of protecting cells from hydrogen peroxide (H2O2)-induced or radiation-induced oxidative stress.
About the Authors
A. L. Popov
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
Russian Federation
Russian Federation
D. D. Kolmanovich
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
Russian Federation
Russian Federation
N. R. Popova
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
Russian Federation
Russian Federation
S. S. Sorokina
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
Russian Federation
Russian Federation
O. S. Ivanova
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
N. N. Chukavin
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Moscow Region State University
Russian Federation
Russian Federation
A. B. Shcherbakov
Zabolotny Institute of Microbiology and Virology
Russian Federation
Russian Federation
T. O. Kozlova
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
S. A. Kalashnikova
40th City clinical hospital
Russian Federation
Russian Federation
V. K. Ivanov
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Russian Federation
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Review
For citations:
Popov A.L., Kolmanovich D.D., Popova N.R., Sorokina S.S., Ivanova O.S., Chukavin N.N., Shcherbakov A.B., Kozlova T.O., Kalashnikova S.A., Ivanov V.K. Synthesis and biocompatibility study of ceria-mildronate nanocomposite in vitro. Nanosystems: Physics, Chemistry, Mathematics. 2022;13(1):96-103. https://doi.org/10.17586/2220-8054-2022-13-1-96-103
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