CeO2-calcein nanoconjugate protective action against H2O2-induced oxidative stress in vitro
https://doi.org/10.17586/2220-8054-2022-13-3-308-313
Аннотация
We studied cerium oxide-calcein nanoconjugate, which is capable of providing intracellular detection and simultaneous inactivation of reactive oxygen species (ROS). The synthesized nanoconjugate is easily uptaken by human mesenchymal stem cells (MSCs) and demonstrates antioxidant properties, protecting cells from H2O2-induced oxidative stress in vitro. Cerium oxide-calcein nanoconjugate neutralizes hydrogen peroxide, meanwhile releasing brightly fluorescent calcein from its surface, which is easily detected by fluorimeter or fluorescent microscope. This nanoconjugate is biocompatible and non-toxic to MSCs in concentrations below 2 mM. Such a theranostic agent can be considered as a promising tool for tracking the redox status of human MSCs in vivo.
Об авторах
N. Chukavin
Institute of Theoretical and Experimental Biophysics; Moscow Region State University
Россия
Россия
A. Popov
Institute of Theoretical and Experimental Biophysics
Россия
Россия
A. Shcherbakov
Zabolotny Institute of Microbiology and Virology
Россия
Россия
O. Ivanova
Kurnakov Institute of General and Inorganic Chemistry
Россия
Россия
A. Filippova
Kurnakov Institute of General and Inorganic Chemistry
Россия
Россия
V. Ivanov
Kurnakov Institute of General and Inorganic Chemistry
Россия
Россия
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Рецензия
Для цитирования:
, , , , , . Наносистемы: физика, химия, математика. 2022;13(3):308-313. https://doi.org/10.17586/2220-8054-2022-13-3-308-313
For citation:
Chukavin N.N., Popov A.L., Shcherbakov A.B., Ivanova O.S., Filippova A.D., Ivanov V.K. CeO2-calcein nanoconjugate protective action against H2O2-induced oxidative stress in vitro. Nanosystems: Physics, Chemistry, Mathematics. 2022;13(3):308-313. https://doi.org/10.17586/2220-8054-2022-13-3-308-313
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