Cellular uptake of FITC-labeled Ce0/8Gd0/2O2-x nanoparticles in 2D and 3D mesenchymal stem cell systems
https://doi.org/10.17586/2220-8054-2024-15-3-352-360
Abstract
Cerium-containing nanoparticles have recently been identified as promising nanozymes for advanced biomedical applications. Additional modification of the core or the surface of CeO2 nanoparticles (CeO2 NPs) provides them with new functionalities, making them a unique theranostic agent. In this study, dextran-stabilized CeO2 NPs doped with Gd (Ce0.8Gd0.2O2−x) were synthesized and further functionalized with fluorescein isothiocyanate (FITC). The synthesized nanoparticles have a high degree of biocompatibility at concentrations up to 5 mg/mL and are readily internalized by human mesenchymal stem cells cultured both in monolayers (2D system) and cellular spheroids (3D system). The functionalization of CeO2 NPs with Gd and FITC dye allows for monitoring their accumulation within organs and tissues using both magnetic resonance imaging (MRI) and fluorescence spectroscopy techniques.
Supporting agencies: The work was supported by the Russian Science Foundation (project No. 22-73-10231, https://rscf.ru/project/22-73-10231/
About the Authors
D. D. Kolmanovich
http://nanojournal.ifmo.ru
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
Russian Federation
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences
Russian Federation
Danil D. Kolmanovich
Pushchino
N. N. Chukavin
http://nanojournal.ifmo.ru
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Scientific and Educational Center, State University of Education
Russian Federation
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Scientific and Educational Center, State University of Education
Russian Federation
Nikita N. Chukavin
Pushchino; Moscow
N. A. Pivovarov
http://nanojournal.ifmo.ru
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Nikita A. Pivovarov
Pushchino; Moscow
S. A. Khaustov
http://nanojournal.ifmo.ru
Scientific and Educational Center, State University of Education
Russian Federation
Scientific and Educational Center, State University of Education
Russian Federation
Sergey A. Khaustov
Moscow
V. K. Ivanov
http://nanojournal.ifmo.ru
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Kurnakov Institute of General and Inorganic Chemistry of the Russian Academy of Sciences
Russian Federation
Vladimir K. Ivanov
Moscow
A. L. Popov
http://nanojournal.ifmo.ru
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Scientific and Educational Center, State University of Education
Russian Federation
Institute of Theoretical and Experimental Biophysics of the Russian Academy of Sciences; Scientific and Educational Center, State University of Education
Russian Federation
Anton L. Popov
Pushchino; Moscow
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Review
For citations:
Kolmanovich D.D., Chukavin N.N., Pivovarov N.A., Khaustov S.A., Ivanov V.K., Popov A.L. Cellular uptake of FITC-labeled Ce0/8Gd0/2O2-x nanoparticles in 2D and 3D mesenchymal stem cell systems. Nanosystems: Physics, Chemistry, Mathematics. 2024;15(3):352-360. https://doi.org/10.17586/2220-8054-2024-15-3-352-360
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