Проникновение наночастиц ФИТС-меченных наночастиц Ce_0.8Gd_0.2O_2-x в 2D и 3D клеточные системы на основе мезенхимальных стволовых клеток

   Церий-содержащие наночастицы являются перспективными нанозимами для передовых биомедицинских применений. Дополнительная модификация ядра или поверхности наночастиц CeO₂ придает им новые функциональные возможности, что делает их уникальным тераностическим средством. В этом исследовании синтезировали декстран-стабилизированные наночастицы CeO₂, легированные Gd, и дополнительно функционализировали их флуоресцеин изотиоцианатом (ФИТС). Синтезированные наночастицы обладают высокой степенью биосовместимости при концентрациях до 5 мг/мл и легко проникают в мезенхимальные стволовые клетки человека, культивируемыми как в монослое (2D-система), так и в клеточных сфероидах (3D-система). Функционализация наночастиц CeO₂ с помощью ионов гадолиния и или ФИТС-метки позволяет контролировать их накопление в органах и тканях с помощью методов как магнитно-резонансной томографии (МРТ), так и флуоресцентной спектроскопии.

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